Antimicrobial resistant gram negative isolates from blood stream infections: A report from BPKIHS

Abhilasha Sharma, Narayan Raj Bhattarai, Basudha Khanal


Objective: To find out resistance pattern and production of ESBL among gram negative blood culture isolates.

Methods: This study was carried out in Microbiology laboratory, BP Koirala Institute of Health Sciences, Dharan, Nepal from July 2011-June 2012. Altogether 150 gram negative bacteria isolated from cases of blood stream infections were evaluated. Isolation, identification of bacteria, determination of antimicrobial susceptibility pattern and detection of ESBL were done following standard protocol.

Results: Resistance to commonly used antimicrobials were noted in varying frequencies. Most of the isolates were resistant to ampicillin (97%) and sensitive to imipenem (98%). Forty seven percent of isolates were ESBL producers and a substantial number of isolates were resistant to at least 3 routinely used drugs.

Conclusion: The study concluded that high resistance was found among the blood culture isolates and ESBL production was seen in a significant number of isolates.


Antimicrobial resistance, Blood culture, Gram Negative Bacteria, ESBL

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