Controlled swelling and in vitro release of insulin from konkoli grafted polymethylacrylaminde hydrogel

Ibrahim Iliya Nkafamiya, Ayodele Akinterinwa

Abstract


The extended study of konkoli grafted polymethylacrylaminde (KG-g-poly (MAAm) hydrogel synthesized in our earlier work from methacrylamide (MAAm) grafted and N,N-methylenebisacrylamide (N,N-MBAAm) crosslinked Konkoli (Maesopsis eminni) galactomannan (KG), is necessary to establish its potential application as an oral insulin delivery system. In this study, the kg-g-poly (MAAm) Hydrogel swelling in typical pH media show an initial rapid swelling before stabilizing. The hydrogel attains its parabolic peak swelling in distill water (pH = 7). Swelling rises from a lower pH medium (pH = 2.2), and in a medium with a slight pH above this (pH = 7.4), swelling starts dropping. Comparing the media for the insulin release studies, the cumulative release drops to its parabolic trough in the distill water medium (pH = 7). A higher cumulative release was recorded at a lower pH (pH = 2.2), and the highest cumulative release was recorded at a higher pH (pH = 7.4). The kinetics and mechanisms of these processes were presented while using the descriptive statistic of regression to fit the results from the processes to a power law. The release of insulin from kg-g-poly (MAAm) Hydrogel was also shown to increase with increase in glucose concentration in the media with an initial rapid release in all solutions, while in a typical intestinal pH media range, higher release at all concentrations was recorded at pH = 6.8.


Keywords


Hydrogels, swelling, insulin, kinetics and mechanisms

Full Text:

PDF

References


Simões S., Figueiras A. and Veiga F. (2012). Modular Hydrogels for Drug Delivery. Journal of Biomaterials and Nanobiotechnology, 3, 185-199 http://dx.doi.org/10.4236/jbnb.2012.32025

Ugwu O.B., Barminas J.T., Nkafamiya I.I., Ayodele Akinterinwa A. (2016). Synthesis and Characterization of Crosslinked Hydrogel of Konkoli (Maesopsis eminii) Grafted Polymethylacrylamide for a Preliminary Study as an Insulin Delivery System. Journal of Biosciences and Medicines, 4, 36-47 DOI: 10.4236/jbm.2016.411005

Kenna N.M. (2015). Stimuli-Sensitive Hydrogel Materials for Sensing and Drug Delivery. PhD Thesis, DCU, 7 - 9.

Singh B, Chauhan G.S, Kumar, S and Chauhan, N. (2007). Synthesis, characterization and swelling responses of pH sensitive psyllium and polyacrylamide basedhydrogels for the use in drug delivery (I), Carbohydrate Polymer 67:190-200

Khodaverdi E., Tafaghodi M, Ganji F., Abnoos K, and Naghizadeh H (2012). In Vitro Insulin Release from Thermosensitive Chitosan Hydrogel. AAPS PharmSciTech, 13(2), 460-466. DOI: 10.1208/s12249-012-9764-9

Singh, B. and Sharma, N. (2008). Modification of sterculia gum with methacrylic acid to prepare a novel drug delivery systems, International Journal of Biological Macromolecules 23: 142-150.

Mahlumba P., Choonara Y.E., Kumar P., Lisa C. du Toit and Pillay V. (2016). Stimuli-Responsive Polymeric Systems for Controlled Protein and Peptide Delivery: Future Implications for Ocular Delivery. Molecules , 21, 1002; doi:10.3390/molecules21081002

Osemeahon, S.A., Barminas J.T., Aliyu, B.A. and Nkafamiya I.I (2008). Development of sodium alginate and Konkoli gum-graft-polyacrylamide blend membrane Optimization of grafting conditions, African Journal of Biotechnology, 7(9): 1309-1313.

Pharmacopoeia of India. III Edition,Vol-II. (1985). Controller of publications, Delhi, Appendex-7, pp A-142

Pourjavadi, A and Mahdavinia, G.R. (2006).Superabsorbency, pH-Sensitivity and Swelling Kineticsof Partially Hydrolyzed Chitosan-g-poly(Acrylamide) Hydrogels, Turkish Journal of Chemistry 30 :595 – 608.

Alfrey, T., Gurnee, E. F., & Lloyd and W. G. (1966). Diffusion in glassy polymers. Journal of Polymer Science Part C, 12, 249–261.

Ritger, P. L.and Peppas, N. A. (1987a). A simple equation for description of solute release I. Fickian and non-Fickian release from non-swellable devices in the form of slabs, spheres, cylinders or discs, Journal of Controlled Release, 5: 23–36.

Ritger, P. L. and Peppas, N. A. (1987b). A simple equation for description of solute release I. Fickian and non-Fickian release from swellable devices, Journal of Controlled Release 5: 37–42.

Singh, B. (2007). Psyllium as therapeutic and drug delivery agent, International Journal of Pharmaceutics 334: 1–14

Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. (1951). Protein Measurement with the Folin phenol reagent, Journal of Biological Chemistry 193: 65

Richter, A., Paschew, G., Klatt, S., Lienig, J., Arndt, K. F., & Adler, H. J. P. (2008). Review on hydrogel-based pH sensors and microsensors. Sensors, 8(1), 561-581.

Zhou HY, Zhang YP, Zhang WF, Chen XG. Biocompatibility and characteristics of injectable chitosan-based thermosensitive hydrogel for drug delivery. Carbohyd Polym. 2011;83(4):1643– 51.

Yin, R.; Wang, K.; Du, S.; Chen, L.; Nie, J.; Zhang, W. Design of genipin-crosslinked microgels from concanavalin A and glucosyloxyethyl acrylated chitosan for glucose-responsive insulin delivery. Carbohydr. Polym. 2014, 103, 369–376. [CrossRef] [PubMed]

Sun, L., Zhang, X., Zheng, C., Wu, Z., & Li, C. (2013). A pH gated, glucose-sensitive nanoparticle based on worm-like mesoporous silica for controlled insulin release. The Journal of Physical Chemistry B, 117(14), 3852-3860.

Shuman C.R. (1988). Diabetes mellitus: definition, classification, and diagnosis. In: Diabetes mellitus (Galloway JA, Potvin JH, Shuman CR, editors), Indianapolis, USA, 2–13




DOI: http://dx.doi.org/10.7439/ijpc.v7i4.4087

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM

Refbacks

  • There are currently no refbacks.


Copyright (c) 2017 International Journal of Pharmaceutical Chemistry

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.