Screening of the Ethnobotanicals against MDA-MB-231 and MCF-7 breast cancer cell lines
AbstractThe present study aims to investigate the therapeutic properties of a library of the medicinal plants for their potential anticancer activity against breast cancer cells. The viability of MDA-MB-231 and MCF-7 breast cancer cells was determined by MTT and trypan blue exclusion assays. The cellular cytotoxicity and the levels of cytosolic enzyme Lactate Dehydrogenase (LDH) were measured by assessing moles of NADH/well/min. Additionally DNA laddering and FACS analysis was done to further investigate the nature of cell death in these cells. Out of the tested plants, only Abrus precatorius L. extract showed a very significant cytotoxic activity on both cell lines. Trypan-blue assay showed the cell viability was significantly decreased in a dose dependant manner in both cell lines after 48 h of treatment. On the other hand, LDH activities in the cultured media were significantly elevated in both cell lines in a dose dependant manner as compared to the control. A prominent DNA laddering pattern in agarose gel was seen, which is an important hallmark of apoptosis. FACS analysis confirmed the apoptosis by the appearance of Sub G0/G1 peak in treated cells as compared to the control. This analysis will facilitate the use of traditional medicine in cancer treatment.
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